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Bromodeoxyuridine, variously abbreviated as Brd U, Bud R, and Brd Urd, is a halogenated thymidine analog that is permanently integrated into the DNA of dividing cells during DNA synthesis in S phase.
Brd U can be immunocytochemically detected in vitro and in vivo, allowing the identification of cells that were dividing the period of Brd U exposure.
In vivo, it has been used to identify the “birthdate” of cells during development, to examine the fate of postnatally generated cells, and to label cells before transplantation, for subsequent identification.
We identify three postnatal proliferative compartments: ventricular, midline and parenchymal.
We find no evidence of postnatal neurogenesis in the pons, but each progenitor compartment produces new astroglia and oligodendroglia; the latter expand 10- to 18-fold postnatally, and are derived mostly from the parenchyma. The nature of postnatal pontine progenitor cells has consequences for pontine gliomagenesis, and for the normal postnatal development and function of this crucial brain region.
To measure proliferation in postnatal development, we injected an age series of CD1 wild-type mice with a single dose of the thymidine analogue Brd U, 100 min before perfusion (Fig. Immunostaining revealed Brd U cells was greater in basis pontis than in any other region of the brainstem (Fig. 3f,g), and also greater in the pontine tegmentum relative to midbrain tegmentum (Supplementary Fig. We observed three main anatomical compartments of Brd U cells were denser in the basis pontis than in the tegmentum (Fig. This greater proliferation in basis pontis coincides with its larger daily per cent increase in volume (Fig. Basis pontis proliferation peaked at 276±15 cells per mm at P4, and declined more than 50% by P12 (Fig. Similarly, proliferation in VZ and midline peaked at P4 with 40.6±3.1 and 16.3±3.7 cells per mm, respectively, and declined more than 50% by P8 and P12, respectively (Fig. There was a significant increase in proliferation density from P0 to P4 in VZ (P=0.0302, unpaired t-test) and basis pontis (P=0.0072), while in tegmentum and midline the increased density of Brd U VZ cells at P28 or above.
These findings show that the pons is the most proliferative postnatal brainstem region; its cell proliferation shows a single postnatal peak, at P4, coincident with the period of fastest growth.Access to society journal content varies across our titles.If you have access to a journal via a society or association membership, please browse to your society journal, select an article to view, and follow the instructions in this box.Pontine neurogenesis occurs prenatally: autonomic nuclei and cranial nerves (V–VIII) derive mainly from rhombomeres 2 to 5 (refs 18, 19), though some tegmental neurons derive from rhombomere 1 (ref.20); the neurons of the basis pontis are born in rhombomeres 6–8 and migrate anteriorly to form the pontine grey nuclei in the territory of rhombomeres 3–4 (refs 20, 21).These data also show that proliferation declines at different rates in pontine VZ, parenchyma, and midline, and at different levels of the brainstem.) 100 min before perfusion, thereby labelling acutely proliferative cells.